分子生物學(xué)技術(shù)在食品微生物檢測中的應(yīng)用
摘 要:本文詳細探討了聚合酶鏈反應(yīng)、實時熒光定量聚合酶鏈式反應(yīng)、擴增片段長度多態(tài)性分析、核酸適配體以及CRISPR-Cas系統(tǒng)等分子生物學(xué)技術(shù)在食品微生物檢測中的應(yīng)用,總結(jié)出這些技術(shù)的具有檢測敏感度高、特異性好、檢測時間短,以及能夠擴大檢測范圍等優(yōu)勢,可為食品安全檢測提供高效、靈活的工具。
關(guān)鍵詞:分子生物學(xué);食品;微生物檢測
Application of Molecular Biology Techniques in Food Microbial Detection
JIAO Xianjun, DONG Guihua
(Department of Microbiology, Zhangqiu Center for Disease Control and Prevention, Jinan 250200, China)
Abstract: In this paper, the application of molecular biology techniques such as polymerase chain reaction, real-time polymerase chain reaction, amplified fragment length polymorphism analysis, aptamers and CRISPR-Cas systems in the detection of food microorganisms is discussed in detail, and it is concluded that these technologies have the advantages of high detection sensitivity, good specificity, short detection time, and can expand the detection range, which can provide efficient and flexible tools for food safety detection.
Keywords: molecular biology; food; microbial detection
近年來,食品安全問題日益突出,已受到消費者的廣泛關(guān)注。(剩余4551字)