重構枯草芽孢桿菌的木糖代謝通路生產乙醇酸

打開文本圖片集

摘 要：本研究旨在獲得一株可利用麩皮生產乙醇酸的食品安全菌。通過同時過表達xdh和yjhG，構建了基因工程菌XDH-YjhG，該菌株能夠利用麩皮生產乙醇酸，產量為3.15 g·L-1。本研究首次證明了可利用麩皮作為碳源生產乙醇酸，為乙醇酸的工業(yè)化生產奠定了基礎。

關鍵詞：枯草芽孢桿菌；麩皮；乙醇酸

Reconstructing the Xylose Metabolic Pathway of Bacillus subtilis to Produce Glycolic Acid

JI Minghua

（Kinry Food Ingredients Co.， Ltd.， Shanghai 200000， China）

Abstract： This study aims to obtain a food-safe bacterium capable of utilizing wheat bran to produce acetic acid. By co-overexpressing xdh and yjhG， a genetically engineered strain XDH-YjhG was constructed， which could use wheat bran to produce acetic acid with a yield of 3.15 g·L-1. This study is the first to demonstrate the use of wheat bran as a carbon source for the production of acetic acid， laying the foundation for the cost-effective industrial production of acetic acid.

Keywords： Bacillus subtilis; wheat bran; acetic acid

乙醇酸（HOCH2COOH）是最簡單的α-羥基酸，別名羥基乙酸或甘醇酸，可廣泛用于食品、醫(yī)藥和化妝品等領域。（剩余3502字）