PCR技術(shù)在食品微生物檢測中的應(yīng)用研究

摘 要：PCR技術(shù)因具有高靈敏度、高特異性和快速性的特點(diǎn)，在食品微生物檢測領(lǐng)域廣泛使用。本文闡述食品中常見的致病微生物、PCR技術(shù)的基本原理、食品中常見致病微生物的PCR檢測方法，探討PCR技術(shù)在食品微生物檢測中的應(yīng)用，并介紹PCR技術(shù)在食品微生物檢測中的發(fā)展趨勢。

關(guān)鍵詞：PCR技術(shù)；食品微生物；致病微生物；實(shí)時(shí)熒光定量PCR；多重PCR

Study on the Application of PCR Technique in Food Microbiological Detection

WANG Di

（Center for Disease Control and Prevention， Wujin District， Changzhou City， Jiangsu Province，

Changzhou 213100， China）

Abstract： PCR technology is widely used in the field of food microbiological detection because of its high sensitivity， high specificity and rapidity. This paper describes the common pathogenic microorganisms in food， the basic principle of PCR technology， the PCR detection method of common pathogenic microorganisms in food， discusses the application of PCR technology in food microbial detection， and introduces the development trend of PCR technology in food microbial detection.

Keywords： PCR technology; food microorganism; pathogenic microorganism; real-time fluorescence quantitative PCR; multiplex PCR

隨著食品工業(yè)的快速發(fā)展和人們生活水平的不斷提高，食品安全問題日益受到政府和公眾的高度關(guān)注。（剩余4174字）