麥類殼多胞斑點(diǎn)病菌的快速檢測(cè)方法研究

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摘要  利用麥類殼多胞斑點(diǎn)病菌β-tubulin一段保守基因序列，設(shè)計(jì)特異性引物進(jìn)行麥類殼多胞斑點(diǎn)病菌的檢測(cè)，并進(jìn)行特異性與靈敏度驗(yàn)證。結(jié)果顯示，該方法特異性強(qiáng)，靈敏度可達(dá)5 pg/μL，適合于麥類殼多胞斑點(diǎn)病菌的快速檢測(cè)。

關(guān)鍵詞  β-tubulin基因；麥類殼多胞斑點(diǎn)病菌；特異性；靈敏度

中圖分類號(hào)  S41  文獻(xiàn)標(biāo)識(shí)碼  A

文章編號(hào)  0517-6611（2024）18-0118-02

doi：10.3969/j.issn.0517-6611.2024.18.025

開放科學(xué)（資源服務(wù)）標(biāo)識(shí)碼（OSID）：

Study on the  Rapid Identification Method of Parastagonospora pseudonodorum

LI  Jin-qing1，2，WANG Ying-chao1，3，SHAO Xiu-ling1 et al

（1.Technical Center of Qingdao Customs，Qingdao，Shandong 266109；2.Technical Center of Yantai Customs，Yantai，Shandong 264000;3.Linyi Vocational University of Science and Technology，Linyi，Shandong 276000）

Abstract  This study utilized partly conserved gene sequence of β-tubulin in Parastagonospora pseudonodorum，designed specific primers for the detection of Parastagonospora pseudonodorum，with specificity and sensitivity verified.The results showed that this method had strong specificity and a sensitivity of up to 5 pg/μL and suitable for rapid detection of Parastagonospora pseudonodorum.

Key words  β-tubulin gene;Parastagonospora pseudonodorum;Specificity;Sensitivity

基金項(xiàng)目  國(guó)家重點(diǎn)研發(fā)計(jì)劃（2021YFD1400100，2021YFD1400103）；青島海關(guān)科研項(xiàng)目（QK202053，QK202016）。（剩余7305字）