蝦源肌球蛋白抗原表位的計(jì)算機(jī)輔助計(jì)算

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摘要：利用在線軟件和線下軟件對(duì)來(lái)源于刀額新對(duì)蝦（Metapenaeusensis）的過(guò)敏原Met el的B細(xì)胞抗原表位和T細(xì)胞抗原表位進(jìn)行預(yù)測(cè)、篩選以及輔助計(jì)算，首先，通過(guò)uniprot蛋白數(shù)據(jù)庫(kù)獲取Met el蛋白質(zhì)氨基酸序列；其次，用ExPASY ProtParam，SignalP-5.0 Server和TMHMM Server v.2.0在線工具對(duì)Met el的理化性質(zhì)、信號(hào)肽與跨膜區(qū)域進(jìn)行分析，用PSIPRED在線工具、SOPMA在線工具和DNAstar軟件聯(lián)合預(yù)測(cè)計(jì)算Met el二級(jí)結(jié)構(gòu)，用Swiss model在線軟件預(yù)測(cè)計(jì)算Met e1三級(jí)結(jié)構(gòu)；再次，用DNAStar線下軟件和IEDB在線軟件綜合預(yù)測(cè)計(jì)算線性B細(xì)胞表位，用IEDB在線軟件預(yù)測(cè)計(jì)算CD4+T細(xì)胞表位和CD8+T細(xì)胞表位；最后將所得結(jié)果進(jìn)行B細(xì)胞表位和T細(xì)胞表的篩選，結(jié)果表明：Metel蛋白的B細(xì)胞表位為15～28，45～49，92～95，125～130，150～153，255～258位氨基酸；T細(xì)胞表位為78～84，223～232位氨基酸.

關(guān)鍵詞：Metel蛋白質(zhì)；B細(xì)胞表位；T細(xì)胞表位；抗原表位篩選

中圖分類號(hào)：R392文獻(xiàn)標(biāo)志碼：A文章編號(hào)：1671-5489（2024）05-1267-07

Computer-Aided Calculation of Antigen Epitopeof Tropomyosin in Shrimp

SHI Yueming'，ZHANG Zhel2，LIU Jianlan3，LIU Minghao3

（1.The Fourth Affiliated Hospital of China Medical University，Shenyang 110032，China;2.Medical Humanities Sciences，China Medical University，Shenyang 110122，China;3.School of Life Sciences，JilinUniversity，Changchun 130012，China）

Abstract：Online and offline software were used to predict，screen and assist in the calculation of the B-cell and T-cell epitopes of the allergen Met el from Metapenaeusensis.Firstly，the amino acid sequence of Met el protein was retrieved from the uniprot protein database.Secondly，the physicochemical properties，signalpeptides，andtransmem brane regions of Met el were analyzed by using online tools ExPASY ProtParam，SignalP-5.0 Server，and TMHMM Server v.2.0.The secondary structure of Met el was jointly predicted and calculated by using the PSIPRED online tool，SOPMA online tool，andDNAstarsoftware，while the tertiary structure of Met el was predicted and calculated by using Swiss model online software.Thirdly，the linear B-cell epitopes were comprehensively predicted and calculated by using DNAStar offline software and IEDB onlinesoftware，CD4+T and CD8+T-cell epitopes were predicted and calculated by using the IEDB online software.Finally，the obtained results were screened for B-cell and T-cell epitopes.The results show that the B-cell epitopes of Met el protein are located at amino acids 15-28，45-49，92-95，125-130，150-153 and 255-258，and T-cell epitopes are located at amino acids 78-84 and 223-232.

Keywords：Metelprotein;B-cellepitope;T-cellepitope;antigen epitope screening

蝦類過(guò)敏原是導(dǎo)致食物過(guò)敏的一個(gè)重要類別，攝入蝦可能導(dǎo)致嚴(yán)重的超敏反應(yīng).原肌球蛋白（Tropomyosin）是主要的熱穩(wěn)定蝦過(guò)敏原.Daul等1研究表明，各品種蝦的主要過(guò)敏原都是一種原肌球蛋白.蝦原肌球蛋白是一種肌纖維蛋白，天然含有2個(gè)亞基（相對(duì)分子質(zhì)量分別為38500，36600），其比例為1：1，可通過(guò)三磷酸腺苷（ATP）誘導(dǎo)的高離子強(qiáng)度組裝成納米纖維[2.蝦原肌球蛋白作為主要的食物過(guò)敏原已被證明具有極高的熱穩(wěn)定性，并能承受食品加工活動(dòng)，可見(jiàn)蝦原肌球蛋白的致敏性較強(qiáng).在棕色蝦（Penaeus aztecus）中鑒定出13種不同的過(guò)敏原，主要有Met el，Penal，Pan s1和Hom al等種類[3.鑒定蝦源肌球蛋白抗原中的表位，對(duì)去除蝦類食品中的過(guò)敏原，開(kāi)發(fā)蝦源肌球蛋白疫苗，進(jìn)行免疫診斷和臨床治療等方面具有重要意義，目前有許多方法可用于研究和確認(rèn)抗原表位，如抗原-單克隆抗體復(fù)合物、蛋白水解或化學(xué)切割片段方法等.但這些方法大多較復(fù)雜，難以執(zhí)行或效率低下，需對(duì)大批潛在的表位候選物進(jìn)行實(shí)驗(yàn)篩選。（剩余7478字）