不同膻味水平的龍陵黃山羊肝組織轉(zhuǎn)錄組SNP分析

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摘要：為加強(qiáng)山羊肉類的質(zhì)量，探究膻味相關(guān)差異基因，對(duì)3個(gè)品種共14只羊的總膻味值進(jìn)行顯著性差異檢驗(yàn)發(fā)現(xiàn)，高膻味水平（總膻味值大于771.845）和低膻味水平（總膻味值低于771.845）龍陵黃山羊總膻味值差異顯著（P

關(guān)鍵詞：龍陵黃山羊;肝臟;SNP;GO富集;KEGG富集

中圖分類號(hào)：S826.8+9         文獻(xiàn)標(biāo)識(shí)碼：A

文章編號(hào)：0439-8114（2022）04-0154-06

DOI：10.14088/j.cnki.issn0439-8114.2022.04.031 開放科學(xué)（資源服務(wù)）標(biāo)識(shí)碼（OSID）：

SNP analysis on liver transcriptome of Longling yellow goat with different odor levels

MA Lin， YANG Ming， WANG Xuan-yi， YE Shao-hui

（College of Animal Science and Tecnology，Yunnan Agricultural University，Kunming  650201，China）

Abstract： In order to enhance the quality of goat meat and explore the difference genes related to the odor， the total of 14 sheep of three breeds were tested for the significance difference of the total odor value. It was found that there was significant difference （P< 0.05） between Longling yellow goat with high（total odor value greater than 771.845） and low（total odor value less than 771.845） levels. Transcriptome sequencing of liver tissues of Longling yellow goats with high and low odor levels was carried out， and SNP analysis was carried out on the obtained data， and SNP genes were analyzed by GO enrichment and KEGG enrichment. The results showed that there were 411 973 SNPs peculiar to Longling yellow goats with high odor level and 444 638 SNPs peculiar to Longling yellow goats with low odor level， and the common SNPs of two were 856 611. GO enrichment analysis showed that SNP gene was enriched in biological process and cell composition. KEGG enrichment analysis showed that SNP gene was significantly enriched in phosphatidylinositol signaling system， complement and coagulation cascade， insulin resistance， splice， autophagy-animal， cofactor biosynthesis， protein processing in endoplasmic reticulum， endocytosis and AMPK signaling pathway. Analysis of SNPs of six genes in fatty acid biosynthesis pathway showed that seven SNPs were located in synonymous coding regions， and two SNPs caused amino acid changes.

Key words： Longling yellow goat; liver; SNP; GO enrichment; KEGG enrichment

羊肉在世界各地廣泛食用，但由于其膻味，在許多亞洲國家并不受歡迎。（剩余8806字）