小麥赤霉菌生防放線(xiàn)菌的篩選鑒定及發(fā)酵條件的優(yōu)化

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【摘要】為篩選出對(duì)小麥赤霉菌有拮抗作用的生防放線(xiàn)菌，本文采用平板畫(huà)線(xiàn)法、對(duì)峙法等進(jìn)行放線(xiàn)菌的篩選。根據(jù)對(duì)小麥赤霉菌的抑菌率大小選定最優(yōu)抑菌效果的放線(xiàn)菌。放線(xiàn)菌液體培養(yǎng)基經(jīng)搖瓶發(fā)酵、正交試驗(yàn)優(yōu)化其培養(yǎng)基配料發(fā)酵條件，確定其發(fā)酵培養(yǎng)基的組成，并利用牛津杯法比較抑菌圈的直徑平均值選擇最優(yōu)的組成比例。

【關(guān)鍵詞】小麥赤霉菌；生防放線(xiàn)菌；抑菌圈；發(fā)酵優(yōu)化；正交試驗(yàn)

【DOI編碼】10.3969/j.issn.1674-4977.2023.05.030

Screening and Identification of Biocontrol Actinomycetes Against Wheat Scab and Optimization of Fermentation Conditions

JIN Keke

（Anhui Zhongqing Inspection and Testing Co.， Ltd.， Hefei 230088， China）

Abstract： In order to screen biocontrol actinomycetes that have antagonistic effects on wheat scab， this article uses plate drawing method， confrontation method， etc. to screen actinomycetes. Select the actinomycetes with the best antibacterial effect based on the antibacterial rate of biocontrol actinomycetes against wheat scab. The liquid culture medium of actinomycetes was subjected to shake flask fermentation and orthogonal experiments to optimize the fermentation conditions of the culture medium ingredients. Determine the composition of its fermentation medium and use the Oxford Cup method to compare the average diameter of the antibacterial zone to select the optimal composition ratio.

Key words： fusarium graminearum； biocontrol actinomycetes； antibacterial circle； fermentation optimization； orthogonal test

小麥赤霉病又稱(chēng)爛穗病、麥秸枯、爛麥頭、紅麥頭、紅頭瘴。（剩余4297字）