HA-RGD對細(xì)胞活性因子修復(fù)功效影響的研究

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【摘要】目的 通過多種檢測方法分析HA-RGD對細(xì)胞活性因子修復(fù)功效的影響。方法 利用一定的比例構(gòu)建HA-RGD、細(xì)胞活性因子組合物1和組合物2，在細(xì)胞水平和人體水平分別評估其對皮膚的修復(fù)效果。結(jié)果 組合物1和組合物2中受試者人體安全斑貼試驗(yàn)結(jié)果均為陰性，未出現(xiàn)不良反應(yīng)，安全性良好；基于細(xì)胞遷移實(shí)驗(yàn)，組合物1和組合物2與空白對照相比，相對遷移平均值顯著升高（P

【關(guān)鍵詞】多氨基酸多糖縮合物；細(xì)胞活性因子；皮膚修復(fù)

中圖分類號：R392 文獻(xiàn)標(biāo)識碼：A 文章編號：1004-4949（2023）18-0070-04

Study on the Effect of HA-RGD on the Repair Efficacy of Cell Active Factor

ZHANG Nan， ZHANG Ting-ting

（Xi’an Bohong Biotechnology Co. Ltd.， Xi’an 710032， Shaanxi， China）

【Abstract】Objective To analyze the effect of HA-RGD on the repair effect of cell active factors by various detection methods. Methods HA-RGD， cell active factor composition 1 and composition 2 were constructed in a certain proportion， and their repair effects on skin were evaluated at the cellular level and human level， respectively. Results Human safety patch test of subjects in combination 1 and combination 2 were negative， no adverse reactions occurred， and the safety was good. Based on the cell migration experiment， the average relative migration of composition 1 and composition 2 was significantly higher than that of blank control （P

【Key words】Polyamino sugar condesate； Cell active factors； Skin repair

間充質(zhì)干細(xì)胞（mesenchymal stem cells，MSCs）作為一種能夠自我更新且具有多向分化潛能的成體干細(xì)胞[1，2]，其在培養(yǎng)過程中會分泌大量的細(xì)胞因子，是除外泌體外另一具有廣泛生物學(xué)活性的小分子蛋白質(zhì)，富含細(xì)胞活性關(guān)鍵的信息傳遞物質(zhì)，這種傳遞物質(zhì)被稱為細(xì)胞活性因子[3]。（剩余6071字）