腎癌組織中肥大細(xì)胞脫顆粒特征及其與血管形成的關(guān)系

打開文本圖片集

Degranulation of mast cells in renal carcinoma and the correlation with angiogenesis

ABSTRACT：Objective To investigate the characteristics of mast cell degranulation in renal carcinoma and the correlation with angiogenesis.Methods Mast cells in human renal carcinoma and adjacent tissues were labeled with Tryptase immunohistochemical staining and the infiltration density was calculated.The degranulation level was evaluated according to the distribution of Tryptase inside and outside the mast cells.Vascular endothelial cells were labeled with CD31 immunohistochemical staining and tumor microvascular density was counted.The difference in the number of cells and degranulation level between renal carcinoma and adjacent tissues were compared.The correlations of degranulation with infiltration density and microvessel density were analyzed.Results A total of 125 renal carcinoma tissues and 52 adjacent tissues were enrolled in the study.Tryptase staining showed that mast cell density in renal carcinoma and adjacent tissues was （2.67±0.22） and （0.63±0.14） cells/HPF，respectively; the positive rate of mast cell degranulation was 70.3% and 34.3%（P＜0.01），respectively.According to the density of mast cells，renal carcinoma tissues were divided into three groups：low，medium and high.The positive rate of mast cell degranulation in the three groups was 42.1%，64.1% and 83.3%，respectively.Tumor microvessel density in the degranulation positive group was significantly higher than that in the negative group [（24.18±1.64） vs.（10.18±1.97） cells/HPF，P＜0.01].Conclusion The level of mast cell degranulation is significantly higher in renal carcinoma than in adjacent tissues，which is associated with infiltration level and angiogenesis.

KEY WORDS：renal carcinoma; mast cell; degranulation; angiogenesis; Tryptase；antivascular-targeted therapy

摘要：目的 探討腎癌組織中肥大細(xì)胞脫顆粒特征及其與腫瘤血管形成的關(guān)系。（剩余7526字）