二肽基肽酶-4在瘢痕疙瘩中的表達情況研究

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[摘要]目的：探討二肽基肽酶-4（DPP4）在瘢痕疙瘩中的表達情況。方法：分別獲取切除術(shù)后的瘢痕疙瘩組織及正常皮膚組織，然后通過蛋白免疫印跡方法、定量聚合酶鏈反應(yīng)，比較二者DPP4基因在蛋白和mRNA水平上表達的差異。結(jié)果：與正常皮膚組織相比，DPP4蛋白在瘢痕疙瘩組織中的表達高于正常皮膚，差異有統(tǒng)計學(xué)意義（P

[關(guān)鍵詞]瘢痕疙瘩;纖維化;二肽基肽酶-4;實時聚合酶鏈反應(yīng);蛋白免疫印跡;基因表達;成纖維細胞

[中圖分類號]R619+.6    [文獻標志碼]A    [文章編號]1008-6455（2022）01-0087-03

Study on the Expression of Dipeptidyl Peptidase-4 in Keloids

LIANG Zhongyao1，CEN Ying2

（1.West China Clinical Medical College of Sichuan University，Sichuan University，Chengdu 610000，Sichuan，China;2.Department of Plastic and Aesthetic Surgery，West China Hospital，Sichuan University，Chengdu 610000，Sichuan，China）

Abstract： Objective To explore the expression of dipeptidyl peptidase-4 in keloids. Methods The keloid tissue and normal skin tissue after resection were obtained， and then the differences in the expression of DPP4 gene between the two were compared by Western blotting and quantitative polymerase chain reaction. Results Compared with normal skin tissue， the expression of DPP4 protein in keloid tissue was higher than that in normal skin， and the difference was statistically significant （P

Key words： keloid; fibrosis; Dipeptidyl Peptidase-4; real-time polymerase chain reaction; blotting western; gene expression; fibroblast

瘢痕疙瘩（keloid）是整形外科中一類常見的皮膚纖維化疾病，發(fā)病機制大多與膠原纖維過度修復(fù)增生有關(guān)，其主要特征表現(xiàn)為高出于皮膚表面、侵襲性生長和容易復(fù)發(fā)。（剩余4722字）