保濕時間對小麥赤霉病抗性鑒定結(jié)果的影響

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摘要 為優(yōu)化黃淮麥區(qū)小麥赤霉病抗性鑒定方法，于2020年在河南農(nóng)業(yè)大學(xué)許昌校區(qū)試驗(yàn)田對4個小麥品種進(jìn)行單花滴注赤霉病抗性鑒定，分析不同套袋保濕天數(shù)對病情嚴(yán)重度的影響，并利用與主效抗病基因Fhb1連鎖的功能標(biāo)記TaHRC-STS對其進(jìn)行分子檢測。結(jié)果顯示：抗病品種‘蘇麥3號’‘寧麥9號’攜帶該基因，而感病品種不攜帶;套袋1～7 d處理均引起赤霉病菌侵染，病情嚴(yán)重度隨著套袋時間的延長而顯著增加，在套袋4～7 d處理下，同一抗、感品種的嚴(yán)重度間無顯著差異;且感病品種4～7 d的嚴(yán)重度顯著高于1～3 d處理（P

關(guān)鍵詞 小麥; 赤霉病; 保濕時間; 禾谷鐮刀菌; 抗性鑒定

中圖分類號： S435.121.45

文獻(xiàn)標(biāo)識碼： A

DOI： 10.16688/j.zwbh.2020704

Abstract In order to optimize the identification method of Fusarium head blight （FHB） resistance in Yellow and Huai River Valley wheat region of China， the FHB resistance of four wheat cultivars planted in experimental field of Xuchang campus of Henan Agricultural University were identified by single floret injection in 2020. The effects of different moisturizing days on the disease severity were analyzed， and then molecular analysis of the four wheat cultivars was performed using TaHRC-STS， a functional marker linked to Fhb1 gene of FHB resistance. The results indicated that resistant cultivars ‘Sumai 3’ and ‘Ningmai 9’ carried Fhb1 gene， while susceptible cultivars did not carry it， and the disease severity increased significantly with the extension of bagging time， and there was no significant difference between the same resistant and susceptible cultivars under bagging for four days to seven days. The severity of susceptible cultivars in 4-7 days was significantly higher than that in 1-3 days， and the evaluation of resistance was also different from that in 1-3 days， which indicated that Fhb1 gene could enhance the FHB resistance. In the identification of FHB resistance， bagging and moisturizing for four days was better in Yellow and Huai River Valley wheat region of China.

Key words wheat; Fusarium head blight; moisturizing time; Fusarium graminearum; resistance identification

小麥赤霉病主要是由禾谷鐮刀菌Fusarium graminearum Schw.引起的小麥真菌病害。（剩余9081字）