Nectin-4在浸潤性膀胱尿路上皮癌中的表達及其臨床意義

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Expression of Nectin-4 in invasive bladder urothelial carcinoma and its clinical significance

SONG Huiru LUO Dan WEN Junxiu NI Lu ZHANG Kexin WANG Qi YANG Liu SONG Xudong DONG Liru

（1.Department of Pathology，The Affiliated Hospital of North China University of Science and Technology，Tangshan 063000; 2.Department of Pathology，School of Basic Medicine Sciences，North China University of Science and Technology，Tangshan 063000; 3.Department of Pathology，The Second Hospital of Shenzhen，Shenzhen 518035，China）ABSTRACT：ObjectiveTo explore the expression of Nectin-4 in invasive bladder urothelial carcinoma （BUC） tissue and its clinical significance，so as to provide reference for clinical diagnosis and treatment of BUC.MethodsNectin-4 expression in 60 cases of invasive BUC and 40 cases of chronic inflammation of bladder mucosa was detected with immunohistochemical staining （IHC） and RNAscope.The results of the two methods were analyzed and compared，and the relationship between the two methods and the clinicopathological characteristics of invasive BUC was discussed.The correlation between the protein expression of Nectin-4 in BUC tissues，human epidermal growth factor receptor 2 （Her-2） and programmed death factor ligand 1 （PD-L1） was analyzed.ResultsThe positive protein expression rates of Nectin-4 detected by IHC were 78.33%（47/60） and 17.50% （7/40） in the invasive BUC group and inflammatory group，respectively，while the positive mRNA expression rates of Nectin-4 detected by RNAscope were 83.33% （50/60） and 12.50% （5/40），respectively.The Kappa values of Nectin-4 in the invasive BUC group and inflammatory group were 0.732 and 0.610，respectively，with general consistency.The protein expression of Nectin-4 in invasive BUC was correlated with muscular invasion，histological grade，vascular thrombus，lymph node metastasis and clinical stage （P<005）.The mRNA expression of Nectin-4 in invasive BUC was correlated with max tumor diameter，muscular invasion，histological grade，vascular thrombus，lymph node metastasis and clinical stage （P<0.05）.The high expression of Nectin-4 in invasive BUC was positively correlated with the expression of Her-2 （P=0.002），but not with the expression of PD-L1 （P>005）.ConclusionNectin-4 is highly expressed in invasive BUC，and is usually associated with the pathological parameters of poor prognosis.Detection of Nectin-4 expression will help to guide clinical diagnosis and treatment.

KEY WORDS：invasive bladder urothelial carcinoma;Nectin-4;RNAscope;immunohistochemical staining;clinicopathological features摘要：目的研究Nectin-4在浸潤性膀胱尿路上皮癌（BUC）組織中的表達及其臨床意義，為BUC的臨床診斷提供參考。（剩余9102字）